Testing for Biological Molecules
1. This question has 4 part(s).
1.1. The Benedict's test for reducing sugars gives a semi-quantitative result when used on samples of reducing sugar solution.
Explain the term semi-quantitative in this context.
1.2. A solution with an unknown concentration of glucose is tested for reducing sugar.
Outline a method for determining the concentration of glucose in this solution.
1.3. Table 1 below gives the experimental protocol for making a serial dilution of a glucose solution.
The concentration of the stock solution of glucose that is to be used is 20 mmol dm-3.
Table 1
Concentration of glucose required / mmol dm-3 | Volume of stock solution required / cm3 | Volume of distilled water required / cm3 | Total volume of solution required / cm3 |
0 | 0 | 100 | 100 |
2 | 100 | ||
4 | 100 | ||
10 | 100 | ||
15 | 100 | ||
20 | 100 | 0 | 100 |
Calculate the missing values and complete Table 1 with your calculated values.
1.4. In a similar procedure to that outlined in part (c), a student wished to make up a solution of glucose of 0.1 mmol dm -3 from a laboratory stock solution with a concentration of 100 mmol dm-3.
The student evaluated two methods of producing this solution, methods A and B as shown below in Table 2.
Table 2
Method A | Method B |
0.1 cm3 stock solution and 99.9 cm3 of distilled water | 10 cm3 stock solution and 900 cm3 distilled water, to make solution X, followed by... |
1 cm3 of solution X and 9 cm3 of distilled water |
Select which method, A or B, would be the better method for the student to follow.
Give a reason for your choice.
Ai এর মাধ্যমে
১০ লক্ষ+ প্রশ্ন ডাটাবেজ
প্র্যাকটিস এর মাধ্যমে নিজেকে তৈরি করে ফেলো
উত্তর দিবে তোমার বই থেকে ও তোমার মত করে।
সারা দেশের শিক্ষার্থীদের মধ্যে নিজের অবস্থান যাচাই
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