1. A scientist used the method below to prepare a sample of organelle F from muscle cells: 

1. Place muscle tissue in a solution that is ice-cold, buffered and has the same water potential as the muscle tissue

2. Place the muscle tissue and solution into a blender; blend for 3 minutes

3. Filter the blended mixture

4. Centrifuge the filtrate at a low speed; a pellet should be seen at the bottom of the centrifuge tube

5. Pour off the liquid from the first centrifuge tube into a second centrifuge tube

6. Centrifuge this liquid at a high speed; the pellet that forms contains organelle F

Explain why the solution used in step 1 had to be ice-cold and have the same water potential as the muscle tissue.