1.1. A student investigated the activity of amylase in the presence or absence of chloride ions. They recorded the rate of formation of maltose against time. The results are plotted in Figure 5.1.

Figure 5.1

Determine the rate of maltose formation in the first 30 seconds of the experiment, for both curves.

Give your answers in cm³min^-1 to 2 decimal places.

1.2. Describe, with reference to Figure 5.1, the role of chloride ions in amylase activity. 

1.3. Suggest how chloride ions might affect the structure of amylase.

1.4. Suggest how the student measured the formation of product in this experiment.