Mode of Action of Enzymes
1. The graph below shows a calibration curve for the absorbance of different concentrations of glucose in solution.
Calibration curves can be used to determine the concentration of unknown samples. A student was wanted to determine how much sucrose they had in an unknown sample using the figure above. To make their results comparable, they first added sucrase to hydrolyse the sucrose into glucose and fructose. They then set their colourimeter to the same settings as the one used in the calibration curve and tested the unknown sample. The sample had an absorbance of 25%.
How much sucrose was present in the unknown solution?
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